Table 1.

Detection of the presence of SARS-CoV-2 using qRT–PCR and CPE on VERO E6 cells.

	Nasopharyngel swaba (Ct)			CPE observed DPI b	Culture fluid from cells with CPEc (Ct)
Sample number	E gene	RdRP gene	N gene		E gene	RdRP gene	N gene	Isolate	TCID50g
1	15.78	18.97	20.95	3d	13.72	17.45	23.52	KMUH-1	104.8
2	36.96	35.93	37.66	-	NDf	ND	ND	-	-
3	36.27	36.45	38.63	-	ND	ND	ND	-	-
4	35.93	36.26	36.98	14e	11.85	16.15	19.73	KMUH-2	105.5

^aRNA extracted from nasopharyngeal swab-UTM.

^bDPI: days post-inoculation.

^cRNA extracted from the culture fluid from cells with CPEs.

^dCPEs were observed in cells inoculated with original nasopharyngeal swab-UTM on day 3.

^eCPEs were observed only in cells with blind passage on day 14.

^fND, not determined.

^gTCID₅₀, Median Tissue Culture Infectious Dose.