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. 2022 Jan 19;12:978. doi: 10.1038/s41598-022-04929-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial organisation of mitochondria in the myocardium (apex) of wild-type (WT) mice. (A) Portion of a serial image taken from an SBF-SEM data stack; red arrows indicate SSM, green arrows indicate IFM. BV blood vessel. (B) Area of a serial image showing a cardiomyocyte nucleus (N) with blue arrows indicating PNM. The dashed green lines indicate how IFM form rows of mitochondria. Scale bar = 5 µm. (C) 3-D Segmentation of part of a nucleus (light blue isosurface) and PNMs at the poles of the nucleus either directly in contact or juxtaposed with the nuclear envelope (individual mitochondria are displayed in a range of colours). (D) A cluster of segmented SSM directly adjacent or touching the sarcolemma (pink isosurface). (E) Examples of segmented IFMs showing that rows are also interconnected. Scale bar = 2 µm. (Fi,ii) Indicates how SSM and PNM follow the topography of the adjacent membrane which may explain why their shapes are generally more irregular than PNM. Scale bar = 2 µm. (G) Examples of WT 3-D reconstructed mitochondria and different shapes. Arrows indicate projections. Scale bar = 1 µm. (H) Analysis of the proportion of each shape type (oval, irregular and mitochondria with a tubular projection) within each WT subpopulation. Scale bar = 1 µm.