Fig. 6. Sodium butyrate inhibits the expression of Kcnj11 and increases [Ca²⁺]_i in rat islet β cells.

A, B Kcnj11 mRNA and protein expressions in rat islets treated with 5 mM sodium acetate (SA), 5 mM sodium propionate (SP), and 5 mM sodium butyrate (SB) for 24 h. C, D Kcnj11 mRNA and protein expressions in INS-1 cells treated with 5 mM SB for the indicated time. E Dispersed rat islet cells were incubated with 5 mM SB for 24 h and intracellular Ca²⁺ concentration ([Ca²⁺]_i) was measured by patch-clamp technique. F Determination of basal [Ca²⁺]_i in E. G The increase of glucose-stimulated [Ca²⁺]_i in E. Data were given as mean ± SD for three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs basal or control (CON) group.