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. 2022 Jan 19;12:1024. doi: 10.1038/s41598-022-04971-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of liver in a porcine model of dietary NAFLD development. Representative liver micrographs, stained with haematoxylin–eosin, from commercial bred swine before (A) and after (B) consuming the steatotic diet for 2 months. Morphometric changes in lipid droplet area expressed as percentage of total liver section (C). Representative liver micrographs before consuming (D) and after consuming the steatotic diet for 2 months (E) using Masson’s trichrome staining. Morphometric changes in fibre area (F), expressed as percentage of total liver section. Representative CD68 immunostaining from liver sections coming from pigs before consuming (G) and after consuming the steatotic diet for 2 months (H). Morphometric changes in CD68 positive areas (I), expressed as percentage of total liver section. Hepatic triglyceride (J) and cholesterol (K) contents before and after consuming the steatotic diet for 2 months. Individual values, means and SD are represented for each group. Statistical analyses were carried out using Mann–Whitney U test. *p < 0.05; **p < 0.01.