Table 1.

Selected hepatic transcripts differentially upregulated in male pigs fed the steatotic diet according to RNAseq at the level of FDR < 0.001 and FPKM ≥ 0.3.

	Gene ID		Symbol	Initial state (FPKM)	Steatosis (FPKM)	Log2 FC
Up-regulated
Apoptosis, cell adhesion	100,038,033	Galectin-3	LGALS3	4.3 ± 1.8	274.9 ± 81.9	6.0
Growth delay and reduction of metastatic potential	100,049,669	Glycoprotein NMB	GPNMB	24 ± 6	776 ± 303	5.0
Activation of macrophages	100,520,753	Macrosialin	CD68	1 ± 0.5	16 ± 9	4.9
Transport of bile acids	100,525,144	Solute carrier family 51 subunit beta	SLC51B	0.3 ± 0.1	8.0 ± 4.8	4.8
Production of interferon-gamma and interleukin-12	397,087	Osteopontin	SPP1	6.1 ± 2.5	151 ± 73	4.6
Down-regulated
Cu and Zn transport	397,123	Metallothionein-3	MT3	8.6 ± 1.6	0.4 ± 0.1	− 4.3
Protein folding	100,514,482	Anterior gradient protein 2	AGR2	8 ± 13	0.6 ± 0.1	− 3.7
Resistance to TNF-induced apoptosis	100,302,365	Phosphatidylethanolamine-binding protein 4	PEBP4	2.6 ± 0.8	0.3 ± 0.3	− 3.0
Nucleotide import	100,156,347	Calcium-binding mitochondrial carrier protein	SLC25A25	64 ± 71	8.3 ± 1.3	− 2.9
Breakdown of phosphoethanolamine	100,519,324	Ethanolamine-phosphate phospho-lyase	ETNPPL	23 ± 16	3.2 ± 1.8	− 2.9
Copper transport	100,037,920	Metallothionein-1E	MT1D	9491 ± 1350	1318 ± 775	− 2.8
Oxidation of squalene	100,113,409	Squalene epoxidase	SQLE	34.6 ± 7	5.1 ± 8.2	− 2.8
Binding of divalent heavy metal ions	102,166,944	Metallothionein-1A	MT1A	4311 ± 913	644 ± 325	− 2.7
Copper ion binding	100,151,998	Monooxygenase, DBH-like 1	MOXD1	1.8 ± 0.5	0.3 ± 0.1	− 2.7
Post-transcriptional repressor	100,157,783	Nanos C2HC-type zinc finger 1	NANOS1	1.3 ± 1.1	0.2 ± 0.0	− 2.6

Data are means ± SD. FPKM, fragments per kilo base per million mapped reads; Log₂ FC, log₂ fold change steatosis/ initial state. Only genes with counts in more than 75% of samples have been taken into consideration.

Biological function obtained from https://www.genecards.org/.