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. 2022 Jan 19;12:1015. doi: 10.1038/s41598-022-04799-7

Table 1.

Results of the original primer blasts against the brown trout genome.

Gene name Ensembl ID Location Ss4R paralogues FSGD paralogues
acana* ENSSTUG00000018681 29 ENSSTUG00000037301 (12)*
bicc2 ENSSTUG00000000142 3
cldn24 ENSSTUG00000028911 27 ENSSTUG00000011864 (9)
csf1r1 ENSSTUG00000006727 3 ENSSTUG00000003249 (13)
coch ENSSTUG00000022865 33
fzd9b ENSSTUG00000041376 13 ENSSTUG00000022387 (26) ENSSTUG00000023903 (15)
kcnk3a ENSSTUG00000002375 1 ENSSTUG00000043204 (35)
melA (opn4b) ENSSTUG00000008119 2 ENSSTUG00000040347 (10)
nptx1** ENSSTUG00000043806 14 ENSSTUG00000021487 (38)**
pax7a* ENSSTUG00000008874 16 ENSSTUG00000004759 (14)* ENSSTUG00000016476 (28)
scarb1 ENSSTUG00000049332 23 ENSSTUG00000023406 (12)
tmem130 ENSSTUG00000003928 18
ednrba ENSSTUG00000006342 31 ENSSTUG00000010807 (21)
mitfa ENSSTUG00000014914 14 ENSSTUG00000034903 (16) ENSSTUG00000032663 (28) and ENSSTUG00000037895 (30)
sox10* ENSSTUG00000036512 32 ENSSTUG00000033003 (1)*
mc1r ENSSTUG00000014295 29 ENSSTUG00000020496 (12)

The Ensembl ID of the best hit and primary assembly location (considered as chromosome number) are reported. For paralogues, duplication events (Ss4R/FSGD), Ensembl IDs, and locations (in brackets next to Ensembl ID) are reported. Primers that match both paralogues are marked with ** next to the gene name; * denotes that one of the primers has one or two nucleotide mismatches to other paralogues. In both cases, both paralogues could be potentially amplified in the first qPCR analyses.