Table 2.
The primers used for qPCR. Rps20 and pgk1 are housekeeping genes.
| Gene symbol | Ensembl ID | Gene description | Forward primer | Reverse primer |
|---|---|---|---|---|
| rps20 | ENSSTUG00000022628 | 40S ribosomal protein S20 | AGCCGCAACGTCAAGTCT | GTCTTGGTGGGCATACGG |
| pgk1 | ENSSTUG00000005810 | Phosphoglycerate kinase | CTCGGTGATGGGGCTTAGG | TCATTGGTGGAGGCGACA |
| acana | ENSSTUG00000018681 | Agrecan core protein-like | TACGAGGCCGGCTACCAC | TGAGCCAGAGCACCGTCATAG |
| bicc2 | ENSSTUG00000000142 | Protein bicaudal C homologue 1-like | GAGGAATCCGGTGGTGACAG | CGTGGCGTAGCTCCTTAACA |
| cldn24 | ENSSTUG00000028911 | Putative claudin-24 | AACCTGCCTGATGTCGTTCC | TTCTCCTGAGGGGTGTTTGG |
| coch | ENSSTUG00000022865 | Cochlin | CACCCATCACCTGTACGACC | GCGTACACCTGAGAACCGAA |
| csf1r1 | ENSSTUG00000006727 | Macrophage colony-stimulating factor 1 receptor 1-like | GTAATTTTGATGATTGCAGATGCG | GGTGGTGCTGGAGATGATGAATA |
| fzd9b | ENSSTUG00000041376 | Frizzled-9-like | CGGCAGGGGCTTTGTAATGA | ACTGTGGCGGTATTCATGCT |
| kcnk3a | ENSSTUG00000002375 | potassium two pore domain channel subfamily K member 3 | CACCGTCTGAAGAAGTGCCT | CACATAATCCCCGAAGCCGA |
| melA | ENSSTUG00000008119 | Melanopsin-A-like, opn4b | ATAACACACCCCAAATACAGGCTA | GCCTCCGTGTCCGTAAATGC |
| nptx1 | ENSSTUG00000043806 | Neuronal pentraxin-1-like | GCGGCTTGATGGGATGGTAT | GTGGGGGAATAACCCGATGG |
| pax7a | ENSSTUG00000008874 | Paired box protein Pax-7-like | ACTGTTCCTTCAGGTGAGGC | CTCCGACTCCACGTCTGAAC |
| scarb1 | ENSSTUG00000049332 | Scavenger receptor class B member 1-like | GTGGAGCAGAGAGGTCCCTA | TCATCAGACGCACTGGGAAC |
| tmem130 | ENSSTUG00000003928 | Transmembrane protein 130-like | ACGCTTTTATCCAGAGCCACG | GAGAAATGGGGAGTCTTCAGC |
| Paralogues | ||||
| mitfa Ch:14 | ENSSTUG00000014914 | Microphthalmia-associated transcription factor-like | TGAGGACAGAAGGGGGTCAT | TGCGATTCTGCCATCGTCTT |
| mitfa Ch:16 | ENSSTUG00000034903 | Microphthalmia-associated transcription factor-like | AACCACGGACTTACCAGCAG | TTTGTCTCTCCTTGGCCATC |
| mitfb Ch:28 | ENSSTUG00000032663 | Microphthalmia-associated transcription factor-like | ATACGATCGCTGTCAACACT | GATCCAGACTTGTCCAGCATA |
| mitfb Ch:30 | ENSSTUG00000037895 | Microphthalmia-associated transcription factor-like | CCACAATCACACGCACGG | TGGTACTTGGTGGGGTTCTC |
| sox10 Ch:1 | ENSSTUG00000033003 | Transcription factor Sox-10-like | GGAGCCCTTTGATGTGAACG | CGTAGGCATAGGGAGAAACAG |
| sox10 Ch:32 | ENSSTUG00000036512 | Transcription factor Sox-10-like | GGAGCCCTTTGATGTGAACG | CGTAGGAATAGGGAGATGCAG |
| pax7a Ch:14 | ENSSTUG00000004759 | Paired box protein Pax-7 | CCTTCAGGTGAGGCTTCATC | CTCCGACTCCACGTCTGAAC |
| pax7a Ch:16 | ENSSTUG00000008874 | Paired box protein Pax-7-like | CCTTCAGGTGAGGCTTCTGT | CTCCGACTCCACGTCTGAAC |
The original primers are listed in alphabetical order according to the gene name. The second half of the table lists the primers for specific amplification of paralogue gene copies.