Whole liver lysates from IDO2 ko, R235W, Y346X, or wt C57BL/6 mice were immunoprecipitated with rabbit anti-IDO2 and then immunoblotted with HRP-conjugated mouse anti-IDO2. (A) A representative blot from total of 3 independent experiments is shown. (B) Graph shows the mean IDO2 protein expression relative to the C57BL/6 control SEM for n=3 samples per genotype. **p<0.01, ****p<0.0001, n.s., not significant