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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Immunol. 2021 Dec 29;208(3):571–581. doi: 10.4049/jimmunol.2100705

Figure 5. Reduced stability of truncated Y346X IDO2 protein.

Figure 5.

293-T-REx™ cells were stably transfected with V5-tagged wt, R235W, or Y346X IDO2. IDO2-transfected cells were cultured in the presence of cycloheximide to inhibit protein synthesis. (A) The cells were harvested after 0, 3, 6, or 9 hours and levels of IDO2 expression in the protein lysates were measured by Western blotting. GAPDH was used as a loading control. (B) Graph shows the percentage of IDO2 expression relative to time 0 for n=3 samples for each cell line/time point. **p<0.01, ***p<0.001