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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Immunol. 2021 Dec 29;208(3):571–581. doi: 10.4049/jimmunol.2100705

Figure 8. Varying effect of R235W and Y346X polymorphisms on binding to IDO2-interacting proteins.

Figure 8.

293-T-REx™ cells, stably transfected with V5-tagged wt IDO2, R235W IDO2, Y346X IDO2 or no IDO2 (none), were transiently transfected with FLAG-tagged (A) GAPDH, (B) Runx1, (C) Mgea5, (D) RANbp10. Lysates were immunoprecipitated with anti-V5 resin and then immunoblotted with anti-FLAG-HRP. Whole lysates were also directly blotted with anti-V5 or anti-FLAG to verify expression of the transfected proteins. Representative blots are shown from a total of 3 independent experiments. Arrows indicate Runx1 and RANbp10 bands.