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. 2021 Jul 20;91(7):1677–1685. doi: 10.1038/s41390-021-01646-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Two subsets of Iba1+ cells were noted in OIR mice: ramified, dendritic-appearing and amoeboid, round-appearing. Certain microglia subset co-stained both Iba1+ and F4/80+. Ameoboid, rounded F4/80+ microglia represented <5% of microglia. RA mice had sparse number of microglia, mainly in a ramified, dendritic phenotype, typical of resting microglia. In OIR mice, Iba1+ microglia was increased, highest at P19, and decreased with age. F4/80 staining was increased at P19 and P24 in OIR mice compared to RA mice, but similar at P32 and P47 in RA and OIR mice. Iba1-stained cells were higher in OIR compared to RA mice at every age tested. The ratio of F4/80 and Iba1 cells was increased at P24, with 30% higher proliferation of Iba1 cells. Yellow arrow shows a magnified view of activated amoeboid microglia in OIR mice that co-stained both Iba1+ and F4/80+. RA room air, OIR oxygen-induced retinopathy, P postnatal day age.