Fig. 1. Cryo-EM structure of the F-encoded OMCC.
a Asymmetric reconstruction (C1) of the OMCCF at 4.22 Å (Supplementary Fig. 2). The C1 structure shows two distinct subassemblies, an outer ring complex (ORC) with 13-fold symmetry and a central cone (CC) with 17-fold symmetry. b A high-resolution map synthesized from the symmetrized ORC and CC maps. ORC is resolved to 3.31 Å with 13-fold symmetry imposed and the CC is at 2.95 Å resolution with 17-fold symmetry imposed (Supplementary Fig. 2). c The atomic models built from (b). d A central slice of the atomic model (c) with different sizes labeled. Subunit colors: TraK (Green), TraB (Blue), TraV (Pink). Structures of the C1, C13, and C17 maps have been deposited with the EMD accession numbers EMD-24768, EMD-24769, and EMD-24770 and atomic models of the C13 and C17 structures have been deposited with the PDB accession numbers 7SPB and 7SPC.