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. 2022 Jan 6;9:816000. doi: 10.3389/fcell.2021.816000

FIGURE 3.

FIGURE 3

The involvement of PI3K/Akt signaling pathway in the Nrf2 activation during the cyclic mechanical stress-stimulated osteogenic differentiation of PDLSCs. (A) LY294002, the PI3K inhibitor, inhibited the level of p-Akt under cyclic mechanical stress based on the western blotting experiments. (B) LY294002 down-regulated the Nrf2 mRNA under cyclic mechanical stress in PDLSCs. (C) LY294002 decreased the cytosol and nuclear protein expression level of Nrf2 under cyclic mechanical stress in PDLSCs. (D) LY294002 inhibited the mRNA levels of osteogenic relative markers (COL1, RUNX2 and OPN) and HO1 under cyclic mechanical stress in PDLSCs. (E) LY294002 inhibited the protein expression levels of osteogenic relative markers (COL1, RUNX2 and OPN) and HO1 under cyclic mechanical stress in PDLSCs. (F) Co-IP experiments showed the protein-protein interaction between Akt and Nrf2 in PDLSCs as immunoprecipitation with anti-Akt antibody and anti-Nrf2 antibody to enrich the protein complex, respectively.