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. 2022 Jan 6;9:816000. doi: 10.3389/fcell.2021.816000

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Copyright © 2022 Xi, Li, Liu, Chen and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The protein-protein interaction between HO1 and SOD2 in PDLSCs under cyclic mechanical stress. (A) Immunofluorescence staining of SOD2 during cyclic mechanical stress in PDLSCs. The scale bars, 100 µm. (B) Cyclic mechanical stress up-regulated the SOD2 mRNA expression in PDLSCs, according to the results of RT-PCR. (C) Cyclic mechanical stress promoted the SOD2 protein expression in PDLSCs, according to the results of western blotting. (D) Co-IP experiments showed the protein-protein interaction between HO1 and SOD2 in PDLSCs as immunoprecipitation with anti-HO1 antibody and anti-SOD2 antibody to enrich the protein complex, respectively.