Figure 4.

Prostaglandin E2 increases when apical NaCl decreases to a minimum of 40 mM in a mPGES1 dependent manner in mouse CCD cell (mCCD-CL1). Each experimental condition was compared to 80 mM NaCl. (A) PGE2 synthesis levels after 24 h stimulation of various apical NaCl concentrations in the apical compartment (left) and basolateral compartment (right). (B) WB analysis of PGE2 synthesis enzymes from same mCCD-CL1 cells. (C) Quantification of the western blots shown in B. (D) mRNA abundance of PGE2 synthesis enzymes normalized to GAPDH. ^*p < 0.05; Statistical significance in A was evaluated by Brown-Forsythe and Welch ANOVA tests and B–D with two-tailed t-test.