Table 1.

Experimental design and drug treatments.

Experiment	Genotype used	Vehicle	Drug	Dose/Delivery	Length of study
HCTZ	Control, CA-SPAK, and SPAK KO	35% DMSO diluted in 0.9% saline	Hydrochlorothiazide (HCTZ) Sigma	25 mg/kg IP	4 days
EP1 antagonist	CA-SPAK	0.9% saline	EP1 antagonist Cayman Chemical SC-51089	6.5 mg/kg IP	4 days