FIGURE 1.

Mapped cell types in human and mouse kidney tissue. In Slide-seqV2, a 10 μm cryosection of kidney tissue is melted onto an array containing 10 μm beads which bind to messenger RNA. Once library preparation is complete, spatially barcoded cDNA corresponding to each bead is assigned a cell identity using Seurat transfer learning. Example mouse, human cortex, and human medulla tissue with all cell types mapped are shown. Individual mappings of podocytes, proximal tubules, and thick ascending limbs are shown for the mouse and human cortex arrays, while vascular smooth muscle, collecting duct principal cell, and thick ascending limb are shown for the human medulla array. Scale bars = 500 μm.