Figure 5.

Moesin^R171W expression is selectively lost in peripheral T cells. 3-4 week-old WT, X-MAID, or MKO male mice were sacrificed and the indicated tissues were harvested and processed for single cell suspensions. Cells were labeled for surface markers, and for intracellular moesin. For blood (A) and spleen (B), populations were gated on live single cells, followed by TCRβ⁺ (T), CD19⁺ (B), NK1.1⁺ (NK), and CD11b⁺Ly6C⁺ (Mono). For thymus (C), populations were gated on live single cells, followed by CD4^–CD8^– (DN), CD4⁺CD8⁺ (DP), CD4 SP, or CD8 SP. (D) Relative Msn (left) and Ezr (right) mRNA expression in CD4⁺ splenic T cells from WT, X-MAID, or MKO male mice. Expressed as the inverse of the Ct of indicated gene subtracted from that of Gapdh. Bars in D represent means. Statistics between WT and X-MAID groups were calculated using a Student’s t test, *p < 0.05.