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Images of IRE11 cells with different levels of R. buchneri-GFPuv infection. Live IRE11 cells were stained with NucBlue Live ReadyProbes Reagent (Hoechst 33342; Invitrogen) and spun onto slides with a Cytospin centrifuge, following Wang et al. (81). Images were captured on an Olympus BX61 DSU Confocal Microscope using a ×60 objective and a double-wavelength filter (DAPI; FITC). DNA shown in blue, GFPuv shown in green.
