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. 2022 Jan 6;8:748427. doi: 10.3389/fvets.2021.748427

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Copyright © 2022 Cull, Burkhardt, Wang, Thorpe, Oliver, Kurtti and Munderloh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rickettsia buchneri inhibits infection and replication of other tick-borne bacteria in tick cell culture. IRE11 cells infected with different levels of R. buchneri-GFPuv were infected with serial dilutions of red fluorescent A. phagocytophilum (A), R. monacensis (B), or R. parkeri (C), and plates were monitored over 14 days for infection and replication, compared to uninfected IRE11 cells. (D) The percentage of IRE11 cells infected with R. parkeri-mKate was assessed by fluorescent microscopy. Measurements not taken on days 6, 9, and 10.