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. 2022 Jan 6;8:748427. doi: 10.3389/fvets.2021.748427

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Copyright © 2022 Cull, Burkhardt, Wang, Thorpe, Oliver, Kurtti and Munderloh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rickettsia parkeri replication in the presence of R. amblyommatis or R. peacockii. Replication of R. parkeri-mKate in tick cells was monitored for 14 days by measuring mKate fluorescence on a microplate reader. (A,B) R. parkeri-mKate replication in IRE11 cells with or without R. amblyommatis at 28°C in a candle jar at challenge doses of 1,000:1 (A) and 10:1 (B); readings not taken on day 8 or 9. (C–E) R. parkeri-mKate replication in IRE11 cells with or without R. peacockii-GFPuv at 28°C in a candle jar at challenge doses of 1,000:1 (C), 100:1 (D), and 10:1 (E). (F) GFPuv fluorescence indicating replication of R. peacockii-GFPuv. Data show mean and error bars standard deviation of three replicate wells. Means were compared to the uninfected control IRE11 using a two-way ANOVA with Dunnett's multiple-comparison test; statistically significant values are marked by asterisks ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. Data are representative of two independent experiments (see Supplementary Figure S4).