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. 2022 Jan 6;8:748427. doi: 10.3389/fvets.2021.748427

Figure 7.

Figure 7

Rickettsia parkeri replication in IRE11 treated with cell-free R. buchneri or R. buchneri lysate. Replication of R. parkeri-mKate in tick cells was monitored for 14 days by measuring mKate fluorescence on a microplate reader. (A–C) R. parkeri-mKate replication in IRE11 at challenge doses of 1,000:1 (A), 100:1 (B), and 10:1 (C) in the presence of cell-free R. buchneri, lysate from R. buchneri-infected IRE11, or lysate from uninfected IRE11. (D–F) R. parkeri-mKate replication in IRE11 treated with lysates from R. buchneri challenged with Rp-mKate for 24, 48, 72, 120, or 168 h. Treated cells were challenged with 100:1 (D) or 10:1 (E) Rp-mKate. Unchallenged control indicates that viable Rp-mKate are present in cell lysate (F). Data show mean and error bars standard deviation of three replicate wells. Means were compared to the uninfected control IRE11 using a two-way ANOVA with Dunnett's multiple-comparison test; statistically significant values are marked by asterisks *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.