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. 2022 Jan 6;8:748427. doi: 10.3389/fvets.2021.748427

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Copyright © 2022 Cull, Burkhardt, Wang, Thorpe, Oliver, Kurtti and Munderloh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of R. buchneri antibiotic cluster genes in response to Rickettsia parkeri challenge. Relative expression of R. buchneri antibiotic cluster genes in response to R. parkeri-mKate challenge over 7 days. Bars show the expression of each gene relative to the control (unchallenged R. buchneri) as the mean and standard deviation of two independent experiments for each qRT-PCR assay. Ct values were normalized to GAPDH expression in infected IRE11 cells. Genes from the polyketide and aminoglycoside clusters are denoted by (A) and (B), respectively. Means were compared to the unchallenged control using a two-way ANOVA with Dunnett's multiple-comparison test.