Table 2.
Genes in Rickettsia buchneri putative polyketide synthesis cluster.
| Label | Locus tag | Accession | Length (aa) | Annotationa |
|---|---|---|---|---|
| HTH domain* | REISMN_01150 (REIS_1819) | KDO03565.1 | 142 | Helix-turn-helix transcriptional regulator |
| Hypothetical | REISMN_01155 (REIS_1817) | KDO03566.1 | 62 | hypothetical protein; type IV pilin |
| ppsE_1* | REISMN_01160 (REIS_1816) | KDO03567.1 | 1448 | Beta-ketoacyl-acyl-carrier-protein synthase I; type I polyketide synthase; erythronolide synthase; acyltransferase domain-containing protein |
| ppsE_2 | REISMN_01165 | KDO03568.1 | 636 | Beta-ketoacyl-acyl-carrier-protein synthase I; type I polyketide synthase; SDR family NAD(P)-dependent oxidoreductase |
| pksL_1 | REISMN_01170 | KDO03569.1 | 522 | Polyketide synthase PksL; SDR family NAD(P)-dependent oxidoreductase; type I polyketide synthase |
| lgrB* | REISMN_01175 | KDO03570.1 | 630 | Linear gramicidin synthase subunit B; non-ribosomal peptide synthetase |
| ppsB | REISMN_01180 | KDO03571.1 | 878 | Plipastatin synthase subunit B; non-ribosomal peptide synthetase |
| ppsE_3 | REISMN_01185 | KDO03572.1 | 554 | Beta-ketoacyl-acyl-carrier-protein synthase I; polyketide synthase |
| lgrE | REISMN_01190 | KDO03573.1 | 239 | Linear gramicidin dehydrogenase LgrE; thioesterase |
| Hypothetical | REISMN_01195 | KDO03574.1 | 265 | hypothetical protein; GNAT family N-acetyltransferase |
| ysdC | REISMN_01200 | KDO03575.1 | 392 | Putative aminopeptidase ysdC; M42 family metallopeptidase |
Asterisks indicate genes with transcription confirmed by RT-PCR.
Locus tags for corresponding genes in the REIS (Wikel) genome are shown in brackets.
a
Bold type indicates annotation recorded in the genome; regular type indicates additional annotations gained through protein BLAST searches.