Table 3.
Genes in Rickettsia buchneri putative aminoglycoside synthesis cluster.
| Label | Locus tag | Accession | Length (aa) | Annotationa |
|---|---|---|---|---|
| PIG-L (PIG-L) | REISMN_01820 (REIS_1505) | KDO03398.1 | 234 | GlcNAc-PI de-N-acetylase; PIG-L family deacetylase |
| btrR_1 (KanB) | REISMN_01825 (REIS_1504) | KDO03399.1 | 421 | L-Glutamine:2-deoxy-scyllo-inosose aminotransferase; DegT/DnrJ/EryC1/StrS family aminotransferase |
| hemL1 (HemL) | REISMN_01830 (REIS_1503) | KDO03400.1 | 420 | Glutamate-1-semialdehyde 2,1-aminomutase 1; aminotransferase class III-fold pyridoxal phosphate dependent enzyme |
| btrB* (Sis6) | REISMN_01835 (REIS_1502) | KDO03401.1 | 518 | Choline dehydrogenase; GMC family oxidoreductase |
| tdh (Tdh) | REISMN_01840 (REIS_1501) | KDO03402.1 | 342 | L-Threonine 3-dehydrogenase |
| kanC* (IstC) | REISMN_01845 (REIS_1500) | KDO03403.1 | 383 | 2-Deoxy-scyllo-inosose synthase |
| luxR (LuxR) | REISMN_01850 (REIS_1499) | KDO03404.1 | 275 | luxR family transcriptional regulator |
| tlcA1 (Tlc2) | REISMN_01855 (REIS_1498) | KDO03405.1 | 521 | ADP/ATP translocase 1 |
| lagD* (ABC) | REISMN_01860 (REIS_1497) | KDO03406.1 | 605 | Lactococcin-G-processing and transport ATP-binding protein LagD; ABC transporter ATP-binding protein/permease |
| hemL (HemL) | REISMN_01865 (REIS_1496) | KDO03407.1 | 435 | Glutamate-1-semialdehyde 2,1-aminomutase |
| Homoserine kinase* (AprU) | REISMN_01870 (REIS_1495) | KDO03408.1 | 342 | Homoserine kinase |
| mde protein (mdlB) | REISMN_01875 (REIS_1494) | KDO03409.1 | 594 | Putative multidrug export ATP-binding/permease protein |
| btrR_2 (btrR) | REISMN_01880 (REIS_1493) | KDO03410.1 | 392 | L-Glutamine:2-deoxy-scyllo-inosose aminotransferase; DegT/DnrJ/EryC1/StrS family aminotransferase |
| Glycogen synthase* (IstM) | REISMN_01885 (REIS_1492) | KDO03411.1 | 422 | Glycogen synthase |
| Methyl transferase (IstN) | REISMN_01890 (REIS_1491) | KDO03412 | 262 | Hypothetical protein; class I SAM-dependent methyltransferase |
Asterisks indicate genes with transcription confirmed by RT-PCR. Protein labels and locus tags for corresponding genes in the REIS (Wikel) genome are shown in brackets.
a
Bold type indicates annotation recorded in the genome; regular type indicates additional annotations gained through protein BLAST searches.