Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 6;12:796916. doi: 10.3389/fmicb.2021.796916

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Liu, Wang, Liang, Dong, Zhang, Liu, Wang, Bi, Liu, Wang, Chen, Liu, Huang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Schematic diagram of CRISPR/Cas12a RR in detecting streptomycin (STR)-resistant Mycobacterium tuberculosis. Schematic diagram of Cas12a RR-mediated STR-resistant M.tb genome detection. Specific CRISPR-guided RNAs (crRNAs) targeting the M.tb rpsl gene were designed for STR-resistant M.tb detection. When CRISPR/Cas12a RR proteins cleave double-stranded DNA with the specific crRNA guide, they induce robust and non-specific single-stranded DNA (ssDNA) trans-cleavage. The ssDNA (sequence TTATT) reporter fluoresces when the quenched fluorescent ssDNA is cleaved (Chen et al., 2018; Xiao et al., 2020). F, fluorophore; Q, quencher.