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Functional verification of the Cas12a RR protein. The Cas12a RR protein recognizes the TTCC (A), CTCC (B), and TCCC (C) targeting sequences. The fluorescent images and fluorescence intensity (A–C) at 15 min of the reaction are shown. (D) Cas12a RR protein recognizes TTCC, CTCC, and TCCC target sequence heat map. Data are presented as the mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
