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. 2022 Jan 6;12:796916. doi: 10.3389/fmicb.2021.796916

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Copyright © 2022 Liu, Wang, Liang, Dong, Zhang, Liu, Wang, Bi, Liu, Wang, Chen, Liu, Huang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of recombinase polymerase amplification (RPA) primers for the rpsl gene of streptomycin (STR)-resistant M. tuberculosis. Optimization of high-efficiency RPA primers used to amplify the rpsl-related gene fragments of STR-resistant M.tb. The amplification efficiency of 16 pairs of RPA primers was evaluated; the fluorescent images (A–D) and fluorescence intensity (E–H) at 15 min of reaction are shown. The content of the STR-resistant M.tb whole-genome template used in the above-mentioned detection reaction is 100 ng. Data are presented as the mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.