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. 2022 Jan 6;12:796916. doi: 10.3389/fmicb.2021.796916

FIGURE 5.

FIGURE 5

Sensitivity and specificity verification of the Cas12a RR detection system. Sensitivity assay using rpsl-crRNAmix to detect gradient-diluted rpsl gene RNA from 10 to 0.01 ng (A,D). Specificity (B,C,E,F) of the Cas12a RR fluorescence method for detecting the rpsl gene of streptomycin-resistant M.tb. The wild-type M. tuberculosis and streptomycin-resistant M. tuberculosis genome nucleic acids were mixed in different proportions to obtain test samples with mutation rates of 100, 10, 1, 0.1, and 0%. The fluorescent images (A–C) and fluorescence intensity (D–F) at 15 min of reaction are shown. Data are presented as the mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.