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. 2022 Jan 6;11:777587. doi: 10.3389/fonc.2021.777587

Figure 2.

Figure 2

Notch2 and Mcl-1 expression in trisomy 12 is modulated by IRF4. (A) Purified trisomy 12 chronic lymphocytic leukemia (CLL) cells were transfected with an empty vector or with IRF4 vector and cultured in complete medium for 24 h. Immunoblots show Notch2 and Mcl-1 expression in three representative trisomy 12 CLL patients. On the right, bar diagrams show the densitometric quantification of Notch2 and Mcl-1 in +12 CLL cells transfected with an empty vector or IRF4 vector for 24 h (*p < 0.05). (B) Trisomy 12 CLL cells were silenced for IRF4 expression for 24 h. Immunoblots show the expression of IRF4 Notch2 and Mcl-1 after transfection with scramble or IRF4 siRNA in three representative samples. On the right, bar diagrams show densitometric quantification of Notch2 and Mcl-1 in +12 CLL cells after 24 h of transfection with IRF4 siRNA for 24 h (*p < 0.05, **p < 0.01). (C) Box plot shows the expression of CD23 measured by flow cytometry after transfection with empty or IRF4 vector for 24 h (**p < 0.01). (D) CD23 expression was measured after transfection of IRF4 siRNA or scramble control for 24 h (*p < 0.05).