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. 2021 Dec 10;17(7):1545–1555. doi: 10.4103/1673-5374.330617

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Copyright: © Neural Regeneration Research

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Time course of BDNF expression in the cortical ischemic penumbra and experimental protocol.

(A) Flowchart of the experiment. (B) Rat that received EA treatment in the awake state. (C) The locations of the Quchi (LI11) and Zusanli (ST36) acupoints. (D) The mRNA expression of BDNF in the ischemic penumbra cortex was measured using quantitative real-time polymerase chain reactions. Data are presented as the mean ± SD (n = 6). #P < 0.05, ##P < 0.01, vs. sham group (one-way analysis of variance followed by least significant difference post hoc test). The experiments were repeated three times. BDNF: Brain-derived neurotrophic factor; EA: electroacupuncture; MCAO: middle cerebral artery occlusion.