TABLE 1.
Reference strains used for evaluation of the species specificity of the 5′ nuclease assay
| Species | Strain designationa | Ct valueb |
|---|---|---|
| Actinobacillus pleuropneumoniae | ||
| Serotype 1 | Shope 4074T | 16.6 |
| Serotype 2 | S 1536 | 16.8 |
| Serotype 2 (Danish isolate) | 4226 | 15.6 |
| Serotype 3 | S 1421 | 17.8 |
| Serotype 4 | M62 | 18.7 |
| Serotype 5A | K17 | 17.7 |
| Serotype 5B | L20 | 19.1 |
| Serotype 6 | Femø | 17.1 |
| Serotype 7 | WF83 | 17.8 |
| Serotype 8 | 405 | 18.1 |
| Serotype 9 | CVJ 13261 | 15.8 |
| Serotype 10 | D 13039 | 17.5 |
| Serotype 11 | 56153 | 19.3 |
| Serotype 12 | 8329 | 17.6 |
| Serotype 13 (biovar 2) | N-273 | 16.0 |
| Serotype 14 (biovar 2) | 3906 | 15.3 |
| Actinobacillus lignieresii | ATCC 49236T | 39.1 |
| CCUG 215 | 38.7 | |
| CCUG 12401 | 40.0 | |
| CCUG 18727 | 40.0 | |
| CCUG 18728 | 40.0 | |
| CCUG 18729 | 38.9 | |
| CCUG 22227 | 40.0 | |
| CCUG 22228 | 40.0 | |
| CCUG 22229 | 40.0 | |
| CCUG 23132 | 40.0 | |
| CCUG 23133 | 40.0 | |
| CCUG 27160 | 40.0 | |
| CCUG 27361 | 40.0 | |
| CCUG 37052 | 36.3 | |
| CCUG 38958 | 40.0 | |
| NCTC 4985 | 40.0 | |
| Actinobacillus ureae | NCTC 10219T | 39.0 |
| P1144 | 36.3c | |
| Actinobacillus capsulatus | NCTC 11408T | 36.4d |
| P1364 | 39.0 | |
| Actinobacillus hominis | NCTC 11529T | 37.0c |
| P1336 | 38.4 | |
| Actinobacillus equuli | NCTC 8529T | 39.5 |
| P1284 | 39.3 | |
| Actinobacillus rossii | ATCC 27072T | 37.7d |
| Actinobacillus suis | CAPM 5586T | 39.6 |
| P1143 | 39.3 | |
| Actinobacillus minor | NM 305T | 40.0 |
| Actinobacillus porcinus | NM 319T | 40.0 |
| 18765-5(T1) | 39.2 | |
| 18870-2(T5) | 39.0 | |
| 18870-2(T7) | 35.8c | |
| 595 | 39.3 | |
| Actinobacillus indolicus | 46KC2T | 40.0 |
| 39Cl | 39.4 | |
| Haemophilus parasuis | NCTC 4557T | 38.0d |
| Mannheimia haemolytica | NCTC 9380T | 38.9d |
| Mannheimia varigena | CAPM 5786 | 40.0 |
| CCUG 16500 | 39.9 | |
| Pasteurella multocida | NCTC 10320 | 38.1d |
| HIM 746–6 | 38.5d | |
| Pasteurella avium biovar 2 | Strain 5 | 39.3 |
| Pasteurella canis biovar 2 | Strain 25 | 39.4 |
| Pasteurella aerogenes | ATCC 27883T | 38.5 |
| Streptococcus suis | 735 | 38.0 |
| 8074 | 39.9 | |
| 24636/74 | 37.7d | |
| Bordetella bronchiseptica | 1317 | 38.2 |
a
ATCC, American Type Culture Collection, Manassas, Va.; CAPM, Collection of Animal Pathogenic Microorganisms, Brno. Czech Republic; CCUG, Culture Collection University of Gothenburg, Gothenburg, Sweden; NCTC, National Collection of Type Cultures, Colindale, United Kingdom.
b
Mean Ct values from three repeats (A. pleuropneumoniae strains) or six repeats (other species).
c
Strains with ΔRn values significantly different from those for the NTCs (P < 0.01).
d
Strains with ΔRn values significantly different from those for NTCs (P < 0.05).