FIG. 6.

Production of extracellular and cell-bound CPSs by C. jejuni grown on minimal medium. (A and B) The gel was stained with either Alcian blue (A) or Alcian blue and silver (B). Lanes 1, 3, 5, and 7, wild-type strain; lanes 2, 4, 6, and 8, kpsM mutant (control); lanes 1 to 6, culture supernatant; lanes 7 and 8, total cell lysate; lanes 1 and 2, no DOC present; lanes 3 and 4, DOC present in the growth medium; lanes 4 and 5, DOC added 3 h before the end of incubation. A sharp ∼18-kDa band is present in all lanes in panel B and comigrates with CPS-PL, corresponding to proteinase K. (C) Effect of phospholipases on the product (CPS) accumulated in the supernatant in the presence of DOC (from panel A, lane 3). Lane 1, CPS-PL (control); lane 2, CPS; lanes 3 and 4, limited and complete hydrolysis with PL, respectively; lane M, prestained markers.