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. 2022 Jan 1;13(2):681–690. doi: 10.7150/jca.62671

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The downregulation of PCAT6 regulates ESCC cells proliferation and migration. (A) The expression of PCAT6 was tested using qRT-PCR after transfection with siRNAs in Eca-109 and Kyse-30 cells. (B) MTT assays were performed to confirm the viability of cell treated with PCAT6 siRNAs. PCAT6 knockdown significantly inhibited the viability of Eca-109 and Kyse-30 cells. (C) Colony formation assays exhibited that PCAT6 knockdown significantly inhibited the proliferation of Eca-109 and Kyse-30 cells. (D) EDU staining assays were conducted to determine cells proliferation following knockdown of PCAT6. The arrow points to the positive cell. Scale bars = 100 μm. (E) The migration abilities of Eca-109 and Kyse-30 cells were examined by transwell assays after transfection, respectively. (Data are mean ± SD. **P < 0.01).