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. 2022 Jan 1;12(3):1303–1320. doi: 10.7150/thno.67702

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Involvement of Rac1 in Shh-MB and limb bud development. (A) Immunohistochemistry staining for PAK1, pPAK1, IFT88, KIF3A, Gli1 and Ptch1 in cerebella tissue slides of GFAP-Cre;SmoM2^+/- and SmoM2^+/- mice. (B) Immunoblotting analyses of PAK1, pPAK1, IFT88, KIF3A, pKIF3A, Gli1 and Ptch1 in cerebella tissues of GFAP-Cre;SmoM2^+/- and SmoM2^+/- mice. (C) Skeletal preparations of the Prx1-Cre;Rac1^f/f and Rac1^f/f mice and their fore- (FL) and hindlimbs (HL) at postnatal day 0 (P0). (D) Immunofluorescence staining for PAK1, pPAK1, IFT88 and KIF3A in limb buds of Prx1-Cre;Rac1^f/f and Rac1^f/f mouse embryos at E10.5. Nuclei were counterstained by DAPI. Bar, 5 μm. (E) Immunofluorescence staining for Gli1 in primary mouse embryonic limb bud fibroblasts of Prx1-Cre;Rac1^f/f and Rac1^f/f. Cytoskeletons were stained by β-actin. Nuclei were counterstained by DAPI. Bar, 20 μm. (F) mRNA levels of Gli1 (left) and Ptch1 (right) in primary mouse embryonic limb bud fibroblasts of Prx1-Cre;Rac1^f/f and Rac1^f/f. N=6. RNA and protein abundance normalized to GAPDH, respectively. **p < 0.01; error bar, SD.