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. 2022 Jan 1;12(3):1247–1266. doi: 10.7150/thno.65516

Figure 2.

Figure 2

(A) Absorption spectra of blank EV, free ICG, free PTX, EV(ICG), EV(ICG/PTX), and SBC-EV(ICG/PTX) samples. (B) TEM images of blank EV and SBC-EV(ICG/PTX). Scale bars indicate 50 nm. (C) Western blot of typical exosomal markers (CD81, CD63, and syntenin) from HEK-293T cells and various EV samples. Calnexin and GAPDH, housekeeping genes, were used as a control. (D) Changes in fluorescence intensity of free ICG, EV(ICG), SBC-EV(ICG), and SBC-EV(ICG/PTX) after incubation in PBS (4 °C) for 14 days. (E) PA amplitude of free ICG, EV(ICG/PTX), SBC-EV(ICG/PTX) at different ICG concentrations. (F) Change in the size of SBC-EV(ICG/PTX) under physiological (i.e., pH 7.4) and acidic (i.e., pH 6.0) conditions, determined by DLS. After 48 h of incubation in pH 6.0 buffers, SBC-EV(ICG/PTX) were destabilized and swollen, and the size distribution increased drastically. (G) TEM images of SBC-EV(ICG/PTX) after incubation in pH 7.4 and 6.0 buffers for 48 h. Scale bars are 50 nm. (H) PTX release profiles from SBC-EV(ICG/PTX) at different pH and US (1 min of irradiation) conditions. (I) PTX release profiles of EV(ICG/PTX) and SBC-EV(ICG/PTX) at different pH conditions after 8 h of incubation (*p < 0.05, **p < 0.01, n = 3).