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. 2022 Jan 21;28(3):348–364. doi: 10.3748/wjg.v28.i3.348

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©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.

This open-access article was selected by an in-house editor and was fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution-NonCommercial-ShareAlike license (CC BY-NC 4.0), which permits others to distribute, remix, adapt, and build upon this work for non-commercial purposes, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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The effect of melatonin on the quantification of immunohistochemistry analysis and the expression of NF-kB with the Western blot technique in the livers of animals that underwent bile duct ligation surgery. The data are expressed as mean ± standard error of the mean. Significant increase in the bile duct ligation (BDL) group in relation to the control (CO) and CO + melatonin (MLT) groups (^aP < 0.001). Significant decrease in the BDL + MLT group in relation to the BDL group (^bP < 0.001); A: Expression of positive pixels; B: Relative ratio (NF-kB/β-actin).