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. 2022 Jan 7;7(2):1682–1693. doi: 10.1021/acsomega.1c04053

Figure 5.

Figure 5

Combination of M1 and Avo B inhibit FAO, a process essential to synergy. FAO-supported respiration was measured as the OCR with palmitoylcarnitine and malate in permeabilized OCI-AML2 (A) or OCI-AML3 (B) cells using high-resolution respirometry. Cells were incubated for 1 h with a 1 μM M1, 2 μM AvoB, the combination, or a vehicle control (DMSO). Data are mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001; one-way ANOVA, Dunnett’s post hoc test. Heat map window concentrations of M1 and Avo B were incubated in TEX (C) or T30R (D) leukemia cell lines, the latter of the two is Avo B-resistant (and subsequently, resistant to Avo B-induced FAO inhibition). Cell viability was measured after 72 h by flow cytometry using 7AAD. All experiments were n = 3. Data are mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001; one-way ANOVA, Dunnett’s post hoc test.