FIGURE 4.

12/15-LOX is required for inflammatory cell recruitment during A. fumigatus lung infection. C57BL/6 WT and 12/15-LOX-deficient (Alox15^−/−) mice were challenged intratracheally with 7 × 10⁷ A. fumigatus conidia. At 6, 12, and 24 h postchallenge, lungs were collected, enzymatically digested, and (A) neutrophils (CD11b⁺ Ly-6G⁺), (B) eosinophils (CD11b⁺ Siglec F⁺), and (C) inflammatory monocytes (CD11 b⁺ Ly-6C⁺ CCR2⁺) were quantified by flow cytometry. The figure represents cumulative data from two independent studies (n = 3–4 mice per group per time point per study). Controls include naive mice (n = 2–3 per group per study). Each circle represents an individual mouse. Line within a given group represents the mean. (D) Thioglycolate-elicited neutrophils or (E) alveolar macrophages from WT and Alox15^−/−-deficient were plated 1:1 with A. fumigatus conidia. Total contents of each well were collected, and fungal killing was assessed by real-time PCR analysis of A. fumigatus 18S rRNA levels. Data are expressed as percentage of fungi killed. The figure illustrates cumulative data from two to three independent studies (n = 1–2 mice per group per study). Each circle represents an individual mouse. Line within a given group represents the mean. (F) Representative H&E-stained lung sections from WT mice (left) and Alox15^−/− mice (right). Original magnification ×10. Scale bar, 1000 μm. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed Student t test).