FIGURE 5.

12/15-LOX is required for neutrophil granulopoiesis during A. fumigatus lung infection. C57BL/6 WT and 12/15-LOX-deficient (Alox15^−/−) mice were challenged intratracheally with 7 × 10⁷ A. fumigatus conidia. (A) Neutrophils (CD11b⁺ Ly-6G⁺) were quantified in blood and (B) bone marrow 6 h after challenge by flow cytometry. The figure represents cumulative data from two independent studies (n = 4–5 mice per group per time point per study). Each circle represents an individual mouse. Line within a given group represents the mean. (C) G-CSF levels were quantified in serum by ELISA. The figure represents cumulative data from two independent studies (n = 4–5 mice per group per time point per study). Controls include naive mice (n = 2–3 per group per study). Each circle represents an individual mouse. Line within a given group represents the mean. (D and E) Neutrophil precursors (five stages differentiated by c-Kit and Ly-6G⁺ expression) were quantified in bone marrow (D) 6 h after challenge or (E) in naive mice by flow cytometry. The figure represents cumulative data from two independent studies (n = 4–5 mice per group per time point per study). Each circle represents an individual mouse. Line within a given group represents the mean. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed Student t test).