FIGURE 6.

Type 17 responses during A. fumigatus lung infection are dependent on 12/15 LOX. C57BL/6 WT and 12/15-LOX-deficient (Alox15^−/−) mice were challenged intratracheally with 4 × 10⁷ A. fumigatus conidia. At 40 h postchallenge, the right lungs were collected, enzymatically digested, and cultured in duplicate for 24 h. (A) IL-17A and (B) IL-22 levels were quantified in coculture supernatants by Luminex-based MILLIPLEX assessment or ELISA. The figure illustrates cumulative data from three independent studies (n = 2–3 mice per group per study). Each circle represents an individual sample. Line within a given group represents the mean. (C and D) At 36 h postchallenge, lungs were collected, enzymatically digested, and (C) iNKT cells (PBS57-labeled CD1d tetramer⁺), and (D) γδ T cells (γδ TCR⁺ and CD3⁺) were quantified by flow cytometry. The figure represents cumulative data from three independent studies (n = 2–3 mice per group per study). Each circle represents an individual mouse. Line within a given group represents the mean. (E) Mice were challenged as described. At 40 h postchallenge, the left lungs were collected and homogenized in 1 ml of PBS supplemented with pro-tease inhibitors. IL-23 was quantified in clarified homogenate supernatants by ELISA. The figure illustrates cumulative data from two to three independent studies (n = 3–4 mice per group per study). Each circle represents an individual mouse. Line within a given group represents the mean. *p < 0.05, ***p < 0.001 (unpaired two-tailed Student t test).