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. 2022 Jan 1;18(3):1134–1149. doi: 10.7150/ijbs.68091

Figure 6.

Figure 6

TBL1X transcription is regulated by Flot2 via c-myc. (A) qRT-PCR results showed that Flot2 regulated the mRNA level of TBL1X. (B) Western blotting results showed that Flot2 regulated the expression of c-myc and TBL1X. (C) After overexpression of c-myc in 5-8FshFlot2 cells, the TBL1X protein level increased compared to 5-8FshFlot2 cells. (D) The c-myc binding sites in TBL1X promoter region were predicted by using JASPAR. (E, F) ChIP assay identified the binding between c-myc and TBL1X promoter DNA fragment. (G) The wild type and mutant pGL3basic-TBL1X promoter-reporter constructs. (H, I) Relative luciferase activities in 5-8F cells treated with si-c-myc (H) and in 6-10B cells treated with oe-c-myc (I) after transfection of pGL3basic-TBL1X plasmids containing wild type or mutant TBL1X promoter region. The results are shown as means±SD.*P<0.05, **P<0.01, ***P<0.001.