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. 2022 Jan 1;18(3):956–969. doi: 10.7150/ijbs.65954

Figure 2.

Figure 2

Sonic hedgehog signaling pathway is activated in the bone tissue of p27 deficient mice. (A) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of differentially expressed mRNAs and (B) the heat map of Shh signaling genes differentially expressed in bone tissue of 3 WT and 3 p27-/- mice. Confirmation of results of microarray using (C) real-time RT-PCR and (D) Western blots. (E) Relative protein expression levels of Shh signaling molecules. Values are mean ± s. e. m. of 3 determinations per group. *: P< 0.05, **: P< 0.01, ***: P< 0.001 compared with WT mice.