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. 2022 Jan 1;18(3):956–969. doi: 10.7150/ijbs.65954

Figure 5.

Figure 5

Deletion of p27 releases Shh transcription suppression mediated via E2F4. (A) A putative E2F4 binding site in the promoter region of the Shh gene highlighted in yellow color. (B & C) Analysis of E2F4 binding to Shh promoter using ChIP assays in wild-type and p27 deficient MEFs. *: P<0.05 compared with wild-type MEFs. (D) Schematic structural diagram of PGL3‐E2F4 promoter and mutant PGL3‐E2F4 Luc reporter plasmid. (E) Luciferase activity driven by the Shh promoter in wild-type and p27 deficient MEFs. Values are mean ± s. e. m. of 3 determinations per group. *: P<0.05; **: P<0.01 compared with the empty plasmid. #: P<0.05; ##: P<0.01, ###: P<0.001 compared with MEFs transfected with the same plasmid.