Table 1.
Primers used in this study for real time RT-PCR
Name | S/AS | Sequence | Tm | bp |
---|---|---|---|---|
p27 | S | GGGCAGATACGAGTGGCAG | 62 | 153 |
AS | TGAGACCCAATTAAAGGCACC | |||
Bmi-1 | S | ATCCCCACTTAATGTGTGTCCT | 60 | 116 |
AS | CTTGCTGGTCTCCAAGTAACG | |||
ALP | S | GGAGCACAGGAAGTTGGGAC | 55 | 393 |
AS | GCGTGCTTGAGCTGAAGCTA | |||
RUNX2 | S | GTGACACCGTGTCAGCAAAG | 55 | 356 |
AS | GGAGCACAGGAAGTTGGGAC | |||
OCN | S | CAAGTCCCACACAGCAGCTT | 55 | 370 |
AS | AAAGCCGAGCTGCCAGAGTT | |||
Col-I | S | TCTCCACTCTTCTAGTTCCT | 55 | 269 |
AS | TTGGGTCATTTCCACATGC | |||
GAPDH | S | TGGATTTGGACGCATTGGTC | 55 | 211 |
AS | TTTGCACTGGTACGTGTTGAT | |||
Shh | S | AAAGCTGACCCCTTTAGCCTA | 60 | 119 |
AS | TGAGTTCCTTAAATCGTTCGGAG | |||
Smo | S | GTGCTGTCTACATGCCCAAGT | 62 | 128 |
AS | GCAACGCAGAAAGTCAGGC | |||
Ptch1 | S | GCCTTCGCTGTGGGATTAAAG | 61 | 118 |
AS | CTTCTCCTATCTTCTGACGGGT | |||
Gli1 | S | CCAAGCCAACTTTATGTCAGGG | 61 | 130 |
AS | AGCCCGCTTCTTTGTTAATTTGA | |||
Gli2 | S | CAACGCCTACTCTCCCAGAC | 61 | 155 |
AS | GAGCCTTGATGTACTGTACCAC | |||
Gli3 | S | CACAGCTCTACGGCGACTG | 62 | 168 |
AS | CTGCATAGTGATTGCGTTTCTTC | |||
Sufu | S | CGGACCCCTTGGACTATGTTA | 60 | 182 |
AS | CTTCAGACGAAACGTCAACTCA | |||
Shh-promoter | S | GGTACCGTGGCCACCTGTGATTATCC | 60 | 280 |
AS | GAGCTCTGAGGACTTGTGAGCTGTCC |