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. 2022 Jan 1;18(3):1120–1133. doi: 10.7150/ijbs.65555

Figure 2.

Figure 2

G6PD is up-regulated in 231-M1 cells compared to 231-C3 cells. (A) Classification of total 54 differentially expressed proteins that were identified through 2D-DIGE proteomics by online Panther system. Three groups of proteins (antioxidant activity, oxidoreductase and response to stimulus) were selected out. (B) The information of five proteins that belong to aforementioned three groups are summarized in a table. G6PD is the only protein that is up-regulated in 231-M1 cells compared with 231-C3 cells. (C) Fluorescent image of 2D-DIGE proteomics gel. G6PD was assigned with ID 11 and was shown as an orange spot on the gel that was enlarged in the upper right corner of the image. (D) The result of G6PD high expression from the proteomics analysis was validated by Western blot analysis. Upper panel shows Western blot analysis of the G6PD and β-actin levels in 231-C3 and 231-M1 cells. Lower panel shows the quantified ratio of G6PD in 231-M1 to 231-C3 cells in proteomics and Western blot result.