Figure 3.

High G6PD expression is positively correlated with poor DOX response in TNBC cells and is associated with low overall survival rate of TNBC patients. (A) Upper panel shows the Western blot result of G6PD level in MCF10A and four TNBC cell lines including MDA-MB-157, BT549, MDA-MB-231, and MDA-MB-468. Lower panel shows IC₅₀ values of DOX in these five cell lines calculated from MTT assay results. (B) Linear regression analysis of G6PD expression level and DOX IC₅₀ values of these cell lines revealed a strong positive correlation with a R-square of 0.993. (C) The comparison of G6PD expression level between TNBC and normal tissues. Data is taken from Curtis et al. (2012), and sample size is indicated in each type. (D) The overall survival probabilities of TNBC patients with high and low G6PD expression in Curtis et al (2012). P-value was calculated using Mantel-Haenszel logrank test.