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. Author manuscript; available in PMC: 2022 Dec 22.
Published in final edited form as: Phys Chem Chem Phys. 2021 Dec 22;24(1):86–97. doi: 10.1039/d1cp04246g

Figure 4. Actin organization, ROS generation and cell mortality of SH-SY5Y cells exposed to Aβ42 oligomers (Aβ-o, 20 μM) in the presence and absence of GQDs.

Figure 4.

(A) Immunofluorescence imaging of actin filaments after a 3 h treatment of Aβ-o (20 μM) and GQDs (2 μg/mL, 10 μg/mL and 50 μg/mL). Actin filaments were stained by phalloidin-iFluor 488 (green). (B) Calculation of the fluorescence intensity of actin filaments according to panel A using ImageJ software. Data points are depicted as mean values (n=3) ± SD, via ne-way ANOVA, ***P < 0.0001 compared with Aβ-o. (C, D) ROS production of SH-SY5Y cells were identified by H2DCFDA staining after 3 h treatment with (C) Aβ-m, Aβ-o and Aβ-f and (D) Aβ-o with the combination of GQDs. H2O2 (200 μM) was used as positive control. (E) Cell mortality after 20 h treatment with Aβ-o and GQDs. Data are shown as mean values (n=3) ± SD, via Two-way ANOVA analyzed at 20 h, *** P < 0.0001 compared with Aβ-o.