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E. canis rP43 expressed in E. coli BL21 with a His6 fusion tag. A Coomassie blue-stained SDS-PAGE gel with uninduced rP43-E. coli (lane 1), rP43-E. coli induced with IPTG (lane 2), and purified E. canis rP43 (lane 3) and the corresponding Western immunoblots with canine anti-E. canis antiserum (lanes 4 to 6, respectively) are shown.
