Table 2.

Nucleotide mutation and amino acid substitution in HPV-31 E6

	HPV-31 E6
No	1	2	3	4	5	6	7	8	9	10	11	12	13
Location	27	69	141	178	190	194	205	219	228	297	321	368	413
Mutation	T–A	C–T	T–C	C–T	A–G	A–G	T–C	A–G	T–C	G–A	A–G	A–G	C–T
Frequency (%)	8.33	27.08	4.17	31.25	4.17	2.08	4.17	16.67	2.08	31.25	27.08	4.17	35.42
Substitution	–	–	–	H60Y	T64A	K65R	F69L	–	–	–	–	K123R	A138V

Compared with the HPV-31 E6 reference sequence (J04353), the mutations are marked with the corresponding bases and amino acid, and those without changes are replaced with a dash (–). No. means the number of nucleotide mutations, Location means the sites of nucleotide mutations, Mutation means the style of nucleotide mutations, Frequency (%) means the percentage of nucleotide mutations, Substitution means the amino acid substitution that occurred by nucleotide mutations